Bloodlines was the third expansion set for Vampire: The Eternal Struggle to be published by White Wolf. As the title suggests, this expansion was the first V:TES set to focus on the bloodlines. Additionally, Bloodlines was the first V:TES set published by White Wolf to consist entirely of new cards, with no reprints from the earlier sets produced by Wizards of the Coast. Bloodlines was also the first set to feature Camarilla, Sabbat and independent vampires in quantity, although the Camarilla is less well-represented than the other groups.
The Ahrimanes, Baali, Blood Brothers, Daughters of Cacophony, Gargoyles, Harbingers of Skulls, Kiasyd, Nagaraja, Salubri, Salubri antitribu, Samedi and True Brujah were introduced to the card game in this expansion, along with their signature Disciplines: Daimoinon, Melpominee, Mytherceria, Obeah, Sanguinus, Spiritus, Temporis, Thanatosis, Valeren, Visceratika, and new forms of Necromancy.